The 25th Massachusetts Infantry lost 7 officers and 154 enlisted men killed and mortally wounded and 169 enlisted men to disease during the Civil War.

1861
September 1 –
October 31
Organized at Worcester
October 31 Moved to Annapolis, Md. and duty there
1862
January 7 Burnside’s Expedition to Hatteras Inlet and Roanoke Island, N. C.; Attached to Foster’s 1st Brigade, Burnside’s Expeditionary Corps
February 8
Battle of Roanoke Island

Commanded by Colonel Upton, the regiment lost 6 enlisted men killed and 3 officers and 41 enlisted men wounded

March 11-13 Expedition to New Berne
March 14 Battle of New Berne and Provost duty there
April 1st Brigade, 1st Division, Dept. of North Carolina
May 15-16 Reconnaissance toward Trenton
May 15 Trenton Bridge; picket and outpost duty
July 24-28 Expedition to Trenton and Pollocksville
July – December Guard, picket and outpost duty at New Berne
November 11 Demonstration on New Berne
December Lee’s Brigade, Dept. of North Carolina
December 10-20 Foster’s Expedition to Goldsboro
December 14 Kinston
December 16 Whitehall
December 17 Goldsboro
December –
October, 1863
Duty at New Berne
1863
January 2nd Brigade, 1st Division, 18th Army Corps, Dept. of North Carolina
March 6-8 Demonstration on Kinston
March 7 Core Creek
March 13-14 Skirmishes at Deep Gully, New Berne
May 20-23 Demonstration on Kinston
May 22 Gum Swamp
June 2nd Brigade, 1st Division, Defenses of New Berne, N. C., Dept. of North Carolina
July 17-20 Expedition to Swift Creek
July 25-31 To Winton
August District of the Pamlico, N. C., Dept. of Virginia and North Carolina
September Defenses of New Berne, N. C., Dept. of Virginia and North Carolina
October Heckman’s Brigade, Newport News, Va., Dept. of Virginia and North Carolina
October 16-18 Moved to Newport News and duty there
1864
January Unattached, United States Forces, Portsmouth, Va., Dept. of Virginia and North Carolina
January 22 Moved to Portsmouth and duty in the Defenses of that city
March 2nd Brigade, United States Forces, Portsmouth, Va.
April 26 Moved to Yorktown; attached to 1st Brigade, 2nd Division, 18th Army Corps, Dept. of Virginia and North Carolina
September Defenses of New Berne, N. C, District of North Carolina, Dept. of Virginia and North Carolina
March 3rd Brigade, 2nd Division, District of Beaufort, N. C., Dept. of North Carolina, then 2nd Brigade Division, District of Beaufort, N. C., Dept. of North Carolina
April 3rd Brigade, 1st Division, 23rd Army Corps, Dept. of North Carolina
May 4-28 Butler’s operations on south side of the James and against Petersburg and Richmond
May 5 Occupation of City Point and Bermuda Hundred
May 6-7 Port Walthal, Chester Station
May 9-10 Swift Creek or Arrowfield Church
May 12-16 Operations against Port Darling
May 14-16 Drury’s Bluff
May 17-28 Bermuda Hundred front
May 28-June 1 Moved to White House, thence to Cold Harbor
June 1-12 Battles about Cold Harbor
June 15-18 First Assault onĀ Petersburg
June 16 –
September 4
Siege of Petersburg and Richmond
August 25 –
September 4
In trenches at Bermuda Hundred
September 4-10 Moved to New Berne, N. C. and duty there
October 5 Non-Veterans ordered home
October 20 Mustered out
December 9-13 Demonstration from New Berne on Kinston
1865
March 3-21 Operations against Goldsboro, N. C.
March 8-10 Battle of Wise’s Forks
March 14 Occupation of Kinston
March 22-23 Moved to Goldsboro and duty there
April 9-13 Advance on Raleigh
April 14 Occupation of Raleigh
May 3-7 Moved to Greensboro
May 12-13 To Charlotte and duty there
July 13-21 Moved to Readville, Mass.
July 28 Mustered out